The sample should be collected in a private room near the laboratory, in order to limit the exposure of the semen to fluctuations in temperature and to con- trol the time between collection and analysis (see Sections 2.2.5 and 2.2.6 for exceptions).
The sample should be collected after a minimum of 2 days and a maximum of 7 days of sexual abstinence. If additional samples are required, the number of days of sexual abstinence should be as constant as possible at each visit.
The man should be given clear written and spoken instructions concerning the collection of the semen sample. These should emphasize that the semen sam- ple needs to be complete and that the man should report any loss of any frac- tion of the sample.
The following information should be recorded on the report form (see Appendix 6, section A6.1): the man’s name, birth date and personal code number, the period of abstinence, the date and time of collection, the com- pleteness of the sample, any difficulties in producing the sample, and the inter- val between collection and the start of the semen analysis.
Collection of semen for diagnostic or research purposes
The sample should be obtained by masturbation and ejaculated into a clean, wide-mouthed container made of glass or plastic, from a batch that has been confirmed to be non-toxic for spermatozoa (see Box 2.1).
The specimen container should be kept at ambient temperature, between
20 °C and 37 °C, to avoid large changes in temperature that may affect the spermatozoa after they are ejaculated into it. It must be labelled with the man’s name and identification number, and the date and time of collection.
The specimen container is placed on the bench or in an incubator (37 °C) while the semen liquefies.
Note in the report if the sample is incomplete, especially if the first, sperm-rich fraction may be missing. If the sample is incomplete, a second sample should be collected, again after an abstinence period of 2–7 days.
*This entire article is taken from the WHO Laboratory Handbook-Examination and processing of human semen, from pages 7. and 8. of the 5th Edition. To the complete of the book and references to the information given http://apps.who.int/iris/bitstream/10665/44261/1/9789241547789_eng.pdf?ua=1